Simulation is an interactive process, so the parameters may be altered, while the simulation is running and the system response may be immediately monitored.
All trials were immediately monitored and scored by a trained observer, using the software program Observer 5.0 (Noldus Information Technology, Wageningen, The Netherlands).
After washing, cells were immediately monitored by the confocal microscope with a 100 × oil-immersion lens (GFP: λexc=488 nm, λem=520 nm; Deep Red: λexc=635 nm, λem=668 nm).
Following pH 5 exposure, the fused cells were immediately monitored by confocal video-microscopy, enabling us to record the time dependence of exchange of these markers between the Golgi populations in the fused cells from the onset of fusion.
When monitoring behavior was included in the model, avoidance of a specific individual was immediately followed by monitoring this individual (see submodel Monitoring below).
A 5-lead ECG was immediately used to monitor cardiac electrical activity, and an Evita 4 ventilator (Dräger, Lubeck, Germany) was used to assist breathing using orotracheal intubation (model number 5-5.5).
Vital signs are also immediately monitored.
The case was immediately considered suspicious and was therefore monitored at least monthly until the boy died.
Mitochondrial Ca2+ uptake was immediately followed in a fluorometer by monitoring the decrease in extra-mitochondrial Ca2+ concentration using the fluorescent probe Calcium-green 5N (Molecular Probes, Eugene, OR, USA) at excitation/emission wavelengths of 505/535 nm, using the FL-solutions software.
He was immediately transferred to the critical monitoring area and put on 100% oxygen via a non-rebreather mask.
