After this, the mixture was immediately cooled down to have the intact cell structure broken down.
Male Wistar rats were anesthetized intraperitoneal with phenobarbital (100 mg/kg) and the intact beating heart was immediately removed under sterile conditions.
On the first noticeable crack in the bone, the test was immediately stopped to allow histologic analysis of the whole intact structure.
I was immediately transported back to childhood, when my sense of wonder was fully intact and I was so willing to be inspired.
One-half of the culture was left intact (A1), whereas the supernatant from the other half was immediately replaced by fresh culture medium following AMF exposure (A2).
Brains were immediately removed, fixed intact in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB), pH 7.2, for 24 hrs and then cryoprotected in 20% sucrose in 0.1 M PB (pH 7.2) overnight.
The intact cysts were immediately placed in an icebox and transported within 3 h to the laboratory at Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Intact rectal biopsies were immediately frozen and stored at −80°C until further processing.
This lysis buffer is ideal for isolation of the linear DNA in an intact form because DNA metabolizing enzymes are immediately inactivated.
The concave corner remains intact during the etching, while convex corners are immediately attacked by the etchant leading to undercut.
