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The right tibia from each rat, collected at sacrifice, was immediately fixed in Burkhardt fixative [42], prior to embedding in methyl methacrylate and sectioning for histomorphometric analyses.
With most tissue samples, part of the tonsil was immediately fixed in Streck Tissue Fixative (STF; Streck Laboratories, La Vista, NE) and then processed for paraffin embedding, sectioning and hematoxylin and eosin (H&E) staining.
This bug was immediately fixed and all stickers were re- moderated.
The muscle biopsy contained within the clamp was immediately fixed in Formalin for subsequent sarcomere length measurement.
After detachment, 10 mL of sample was immediately fixed with 2% formaldehyde (final concentration) and cooled at 4 °C for later direct counting of cells.
One set of coverslips was immediately fixed and stained as indicated above.
Similar(22)
Cells were immediately fixed in a fixative solution as methanol acetic acid (6:1) 2 times.
Skin samples for immunostaining were immediately fixed in cold Zamboni's fixative and kept at 4°C overnight.
Samples were immediately fixed in 10%% neutral-buffered formalin.
PBMCs were immediately fixed thereafter using fixation/permeabilization buffer (True-Nuclear-kit, BioLegend) according to manufacturer's instructions.
Cells were immediately fixed with 4% formaldehyde in PBS and permeabilized on ice with BD Phosflow Perm Buffer III BD BiosciencesSan Jose, CA CA, USA).
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