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The resulting wet film was then heat-treated at 90 °C for several seconds and was immediately dipped into the MAI solution and kept for 90 or 150 s to study the effect of dipping time.
The cut end was immediately dipped into a 1.5-mL plastic vial containing distilled water.
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After etching, the samples were immediately dipped into 50 wt.% HNO3 to dissolve the as-generated Ag dendrites.
The freshly sampled tissues were immediately dipped in liquid nitrogen and stored at -80°C until RNA extraction.
Specimens from cancer tissue and adjacent normal tissue were obtained during the surgery and were immediately dipped in 10% formalin for immunohistochemistry analysis.
In all cases, white roots were immediately dipped in liquid nitrogen to prevent degradation and stored at -80°C until RNA extraction.
The swab specimens were immediately dipped into a sterile tube containing two drops of sterile physiological saline and taken to the Microbiology Laboratory of Bahir Dar Regional Health Research and Laboratory Center within five minutes of collection.
Following the last wash, slides underwent secondary fixation in 7% formaldehyde in 1× egg buffer for 5 min and were immediately dipped in 2× SSCT to wash off fixative.
The slide is then immediately dipped into both a methylene blue nuclear stain for 15 seconds followed by a pink counterstain for 15 seconds.
The mice were sacrificed after 24 h post-treatment and the lungs were removed and immediately dipped into 4% paraformaldehyde solution in PBS for 1 3 days.
The slide was snap-frozen by dipping in liquid nitrogen and the coverslip was immediately removed.
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