Stained cells were immediately acquired on a FACScalibur (Becton Dickinson) and analyzed with Flow Jo software.
Stained cells were immediately acquired on a LSR II flow cytometer (BD Biosciences), configured to detect 13 parameters.
In contrast, if SWATH maps are immediately acquired after collection, they can be matched and compared between each other, thus allowing for interrogation of diagnostic profiles.
Cells were immediately acquired on a BD LSR II with a High Throughput Sampler delivery system and analyzed with FACSDiva 6.1 software (all BD Biosciences, San Jose, CA, USA).
Cells were immediately acquired on a flow cytometer (LSRII; BD Biosciences) and analysed using a software package (FlowJo; Treestar, Ashland, OR, USA).
The cells were then washed twice with HBSS and fluorescence levels were immediately acquired with excitation and emission wavelengths set at 535 and 590 nm, respectively, for red fluorescence, and 485 and 535 nm, respectively, for green fluorescence.
After removal of the microelectrode from the injected cell, images were immediately acquired using a CoolSNAP-HQ2 CCD (charge-coupled device) camera (Photometrics) attached to a Nikon inverted microscope equipped with 10×dry objective (NA 0.3) and FITC filter sets.
If a pair of traits i and j were acquired simultaneously, we would expect γ ij and γ ji to both be higher than expected in the non-contingent case (as j should always appear to be immediately acquired after i and vice versa).
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