Root tip regions were imaged utilizing the same microscope and camera used previously for transient GFP detection but the robotics components were disabled.
A single living cell (PC12), cultured on a polystyrene dish and an albumin island immobilized on glass, were successfully imaged utilizing the differential standing approach (d-SA) mode of a scanning ion conductance microscope (SICM).
Multiple Z-planes of the organoids were subsequently imaged utilizing an IX81 microscope (Olympus) running Slide Book 5.0 software (Intelligent Imaging Innovations).
The bubble raft was imaged from the edge utilizing OCT and a CCD camera was used with normal orientation to the raft as shown in Fig. 3.
The lower clouds, the lower atmosphere and the surface are imaged by utilizing near-infrared windows.
The megalin-stained area in each image was determined utilizing computer-assisted image analysis software, Simple PCI (Hamamatsu Co, PA USA).
The stained area in each image was quantified utilizing computer-assisted image analysis software (Simple PCI, Hamamatsu).
The quantitation of band intensities was performed on scanned images utilizing ImageJ Software.
Mice were then injected IP with 15 mg/ml D-luciferin in sterile PBS (150 mg/kg) and exactly 14 min after injection of D-luciferin, a bioluminescent image was captured utilizing an exposure time of 1 minute.
Cell images were taken utilizing a Zeiss Imaging System (Carl Zeiss MicroImaging, Inc., Thornwood, NY, USA).
For all immunolabeling studies, assessment of the levels of immunoreactivity was performed utilizing the Image-Pro Plus program (Media Cybernetics, Silver Spring, MD, USA).
