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In order to test whether differences between dynamin1 and dynamin2 during clathrin-mediated endocytosis could be observed, each was imaged simultaneously with clathrin by live-cell TIR-FM in PC12 cells.
Perfusion was imaged simultaneously.
The contralateral left knee was imaged simultaneously as an internal control.
The fluorescence of GFP and FM4-64 was imaged simultaneously by using the exciting laser line 488 nm, but separate emission detectors (490 – 543 nm and 667 – 746 nm, respectively).
The emission pathway for both fluorescent reporters was imaged simultaneously with an image splitter (Dual View, Optical Insights, Santa Fe, NM, USA) creating side-by-side images of both emission wavelengths on the chip of a charge-coupled device (CCD) camera (Cascade II 512B, Roper Scientific, Trenton, NJ, USA).
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Three independent sets of experiments were conducted, and in each case all three genotypes were imaged simultaneously using identical imaging conditions.
This approach allowed all four phantoms to be imaged simultaneously.
(d) Vessels highlighted with QD (red) were imaged simultaneously with the second harmonic generation signal from collagen (blue).
These measurements reveal coexistence of three symmetrically equivalent in-plane orientations with respect to the substrate, each of which is imaged simultaneously within a single area.
Ten patients were imaged simultaneously with 131I-MIBG and 111In-pentetreotide (as a surrogate for 90Y-DOTATOC) on days 1, 2, and 3 post-administration.
Both calves can be imaged simultaneously in the coronal plane following gadolinium injection, and when used, should be performed first in a lower limb CE-MRA.
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