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Tissue was homogenized at 4°C and the resulting homogenate centrifuged for 12,000 rpm for 15 min at 4°C to remove cell debris.
For the primary emulsion, 250 μl of the porogen (phosphate buffered saline (PBS); Gibco, UK) was added to the PLGA/DCM, and the PLGA/DCM/PBS was homogenized at 9000 rpm for 2 min using a Silverson L5M homogenizer.
The geometry of the textile architecture was incorporated in a mesoscale finite element (FE) model, while the H3DTC was homogenized at the macroscale.
The mixture was homogenized at a speed of 11,000 rpm for 1 min.
Sample (2.0 g) was mixed with 20 ml of cold 40 mM phosphate buffer (pH 6.8), and the mixture was homogenized at 13,500 rpm for 10 s.
Ground sample (4.5 to 5.5 g) was homogenized at a speed of 13,500 rpm for 2 min in 11 ml of chloroform/methanol (2 1, v/v).
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After the incubation period, the spent media were collected and the liver slices were homogenized at 4°C, the homogenates were sonicated for 4 seconds and centrifuged at 4°C.
After freeze/thaw three times using liquid nitrogen, worms were homogenized at 4°C, using a glass homogenizer and centrifuged at 4°C, 2000 rpm for 15 minutes.
0.5 g of Frozen human or animal brains were homogenized at low speed (Cole-Palmer homogenizer, LabGen 7, 115 Vac) in 1×STEN buffer and centrifuged at 1000 × g for 10 min to isolate the supernatant from the pellet.
Tissue samples were homogenized at 10,000 rpm using a polytron homogenizer in TriPure Isolation Reagent (Roche Diagnostics) and total RNA was isolated according to the manufacturer's protocol.
The mixtures were homogenized at 10,000×g for 2 min using IKA LABOTECHNIK homogenizer (model T25 basic, ULTRA TURREX®, Selangor Malaysia).
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CEO of Professional Science Editing for Scientists @ prosciediting.com