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The reaction was halted using 50 mM Tris/HCl pH 7.5.
The DAB reaction was halted using PBS, followed by three 10 min PBS rinses.
After fixation, the crosslinking reaction was halted using 0.125 M glycine for 5 minutes while rocking at room temperature.
Outlier analysis was conducted for F6-generation individuals (i.e. first generation after the selection was halted) using the Fdist method (Beaumont and Nichols 1996) implemented in software LOSITAN – Selection Workbench (Antao et al. 2008).
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Transposition reactions were halted using QG buffer (Qiagen) and fragmented cDNA was purified on AMPure XP beads.
However this approach can induce a stress response within the cell and further transcription must be halted using actinomycin D. This combination was used by Bloethner et al. (2008) in an approach called Gene Identification by NMD Inhibition.
The assay was then halted using 25 μl of Beadlyte Stop Solution.
In addition, we examined CDI treatment factors including whether antibiotic use was halted, probiotic use and the type of anti-CDI drugs used (ie, vancomycin and metronidazole).
Clemens's trial on lying to Congress was halted because prosecutors used inadmissible evidence.
About the same time (1959) commuter train service to the town was halted, a land-use disaster.
In 1804, coinage of silver dollars was halted; the last date used during regular mint production was 1803.
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