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The reaction was halted by adding 20 µl of formamide buffer (90% formamide, 1× TBE, bromophenol blue) and immediately transferred to 95°C for 5 min. The digested samples were resolved in duplicates (to enable C- and G-strand specific hybridization in parallel) on a gradient (4 8%) polyacrylamide gel containing 8 M urea in 1× TBE.
The reaction was halted by adding 2× SDS sample buffer.
The reaction was halted by adding 1N sulfuric acid.
The reaction was halted by adding 0.1 ml of 1 M citric acid.
The cDNA synthesis was halted by adding 3 μl 0.2 M EDTA (pH 8.0).
The reaction was halted by adding 1 N HCl (Sigma) and the optical density read at 450 nm.
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The bloodbath was halted by Vietnamese invasion.
This crack growth by blunting-resharpening accelerated with rising ΔK and was halted by an overload.
The study was halted by the federal authorities.
Their work was halted by World War I.
Fighting was halted by the Armistice of Mudros (Oct . 30 , 1918
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