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In the presence of PEG as a dispersant, Boc-amino acid was ground with zirconia beads in water using a planetary ball mill for 2 hours at room temperature.
With the rest part of the sample, easily identified organic matter (roots, stems, leaves) was removed and air-dried in open for 7 days and, later, the soil was ground with a wooden hammer and sieved through a 2-mm diameter mesh.
A portion of each soil sample was ground with an agate grinder to pass through a 0.15 mm nylon sieve.
Each sample was ground with a porcelain mortar and pestle.
Freeze dried biomass was ground with a pestle in a mortar.
The dried ZrP was ground with a mortar and pestle into fine powders.
Mn oxide was ground with a mortar and pestle before sieving (150-µm mesh) to achieve a uniform particle size.
Before coating, the surface of the metal was ground with grade 400 2000 emery papers gradually, then degreased in acetone, ethanol, and distilled water for 10 min, respectively.
Therefore, for extraction of the resistance-activating substances, mycelium mat was ground with liquid nitrogen and the resulting powder was extracted separately with various solvents.
Starchy endosperm (5 mg) was ground with a mortar and pestle and the powder was boiled in 1.5 ml of methanol for 10 min.
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If the tissue was ground with a mortar and pestle and does not need additional grinding, continue with the next step.
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