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To ensure that this mutation was genuinely present in the original sample, PCR and sequencing of the gene region around this mutation were performed using the original ClbiNPV DNA.
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Maybe she'd still receive a sense of love and community from more meaningful social calls when you're able to be genuinely present instead of busy with chores.
And they will feel -- especially in this culture of distraction -- that you are genuinely present with them in the moment.
Either there is a similar contaminant, or the gene is genuinely present in the dinoflagellate genome.
The proportion of APC, β-catenin, GSK3β and CK1α, as well as PP2A (not shown), detected in the PS fraction was consistently greater than that of nucleoporin (Fig. 4C, D), demonstrating that they are genuinely present, although not necessarily enriched in pseudopodia.
No PCR products were generated from such mixed templates, suggesting that PCR templates for the recombinant variants were genuinely present in the hybrid scallops.
Several of these sequences were cloned from cDNA in order to ensure that they were genuinely present in G. pallida and for further functional analysis (below).
This suggests that mutation artefacts were not introduced during the methodology and that all mutations subsequently detected were genuinely present in the Barrett's tissues.
This implies that the total residual confounding (including residual confounding from unmeasured factors) likely biases, but only very modestly, towards observing a larger effect size for statins than is genuinely present.
While it is possible that the suspect taxa are genuinely present in these samples, in many cases they are biologically unexpected: for example, rhizosphere-associated bacteria that have been implicated in human disease [ 27, 44].
We used PCR amplification to amplify this region from four of the C. jejuni isolates testing positive for SSH629-D8, including strain 629, and confirmed that this mutation is genuinely present and not an artefact of the SSH procedure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com