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The omovertebral bar was gently detached from its insertion to the cervical spine.
The retina was gently detached and separated from the underlying retinal pigment epithelium and cut into pieces in a Ringer solution to release individual rod cells.
A sagittal skin incision from behind both eyes to the occipital bone was made and the periosteum was gently detached from the underlying bone to allow visualization of bregma.
Afterward, it was gently detached from the glass plate using tweezers and placed in a fresh water bath of 5 L laboratory water type 1 for full hydration for 24 h.
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After growing until 70 to 80% confluence, the cells were gently detached using 0.05% ethylenediaminetetraacetic acid (EDTA)/phosphate-buffered saline (PBS), washed twice, resuspended in 10% FCS/RPMI and frozen down in liquid nitrogen.
They were gently detached with a rubber policeman and resuspended in 800 µl of homogenization buffer (10 mm triethanolamine, 10 mm acetic acid, 250 mm sucrose, 20 mm N-ethylmaleimide, 1 mm EDTA, and a mixture of protease inhibitors, pH 7.4).
To determine the success of vertical transmission in vegetative tillers (F1 generation), four tillers were gently detached from 10 E+ plants (maternal environment: 6 and 4 from meadows and river banks, respectively) and from 10 ME+ plants (maternal environment: 4 and 6 from meadows and river banks, respectively) growing in the common garden in 2007.
Cells were gently detached using repeated aspiration and expulsion of cold PBS.
HEK293 cells were gently detached from plates after short incubations (60″) in 1X Trypsin-EDTA solution (Life Technologies).
The lifted chondrocyte sheet edges promptly attached to the overlaid supporting membrane, and the cell sheet and PVDF membrane film were gently detached from the UpCell dish.
Cells were gently detached off the tissue culture surface with enzyme-free cell dissociation buffer (Invitrogen) and washed once with complete medium.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com