Sentence examples for was gently added from inspiring English sources

While it was agitated by stirrer, 3.5 g 12-TSA·7H2O 12-TSA·7H2Oadded to it.

An equal volume of cold water was gently added and the product cooled in a refrigerator for 30 min.

A seed solution of 120 μL was gently added into the resultant solution and was continuously stirred for 2 min, and then the reaction mixture was stored at 30°C for at least 3 h without agitation.

After incubation, medium was gently added to each dish as to not dissociate aggregates.

In method B, PBS was gently added and replaced three times with a standard pipette.

The maintenance medium described later was gently added on the top of the scaffold to induce gelation.

Cells (in HBSS +10% FBS) were gently added on top of the Percoll layers (max 40*106 cells) and centrifuged at 18 22°C for 45 minutes at 1750 g without brake.

Cell−backpack aliquots at the indicated ratio were gently added to 18 mL pH 7.4 PBS in a quartz cuvette.

After aspiration of medium and washing of the cells with 1x PBS (2 × 1 mL), these complexes were gently added to respective wells and the plate was kept in an incubator at 37°C for 3 h.

Tau-seeds (1 µL; 333 µM) were gently added on top of hippocampal slices at DIV3 and DIV6, and slices were analyzed electrophysiologically, biochemically and immunohistologically 10 days after seeding.

Then the medium was aspirated, the cells were washed with phosphate buffer saline (1x PBS, 2 × 200  μL), and the pDNA complexes of TP1 to TP5 were gently added, prepared at w/w ratio of 1.6, 2.6, 3.3, 5.0, and 6.6, bPEI at w/w ratio of 1.6, and Lipofectamine, prepared following the protocol provided by the manufacturer, onto the cells.