A pectinolytic industrial yeast strain of Saccharomyces cerevisiae was generated containing the S. cerevisiae endopolygalacturonase gene (PGU1) constitutively expressed under the control of the 3-phosphoglycerate kinase gene (PGK1) promoter.
To allow for a direct comparison between the processes at both the calcite and α-quartz interface a periodic cell (dimensions 24.906 × 40.00 × 19.806Å) was generated containing a slab of both minerals.
A Noccaea caerulescens genomic library was generated, containing 36,864 fosmid pCC1FOS™ clones with insert sizes ∼20 40 kbp, and screened with a PCR-generated HMA4 genomic probe.
To target the URA3 gene to the AAC2 locus, a URA3 cassette was generated containing DNA fragment flanked by sequence homologous to the ∼200 bp immediately up- and downstream of the AAC2 ORF [25].
A fusion image was generated containing all spot positions.
For PbΔ slarp -a, the DNA-construct pL1740 was generated containing the positive/negative selectable marker cassette hdhfr / yfcy.
Firstly, a text file was generated containing the coordinates of all DHL92 annotated genes as 'contig_or_scaffold_name:star_coord-end_coord gene_name'.
An expression table was generated containing square root transformed tpm expression levels for each unique individual isomir transcript.
For PbΔ b9 Δ slarp, the DNA-construct pL1740 was generated containing the positive/negative selectable marker cassette hdhfr/yfcy.
To select the best RBFNN, a grid for training the net was generated, containing different values of spread and different precision values (goal error).
