An air flow was generated by using a ventilator.
The transdermal spray was generated by using a film forming polymers like Eudragit RLPO and ethyl cellulose (1 2 ratios) along with eutectic camphor: menthol (1 1) mixture used as a penetration enhancer.
An arbitrary direction of a magnetic field vector was generated by using a three-axis Helmholtz coil.
The inu1 deletion strain was generated by using a Cas9 plasmid (E02-026) containing gRNA-1 and gRNA-2 target sequences (Table 2 and Fig. 1a).
Polyadenylated cyclin D1 mRNA was generated by using a poly(A) tailing kit (Ambion).
Complementary DNA (cDNA) was generated by using a TaqMan Reverse Transcription Kit (Applied Biosystems).
First strand synthesis of the cDNA was generated by using a clamped dT primer, which is designed to attach to the poly-A junction of the mRNA.
Capped cyclin D1 mRNA was generated by using a mMESSAGE mMACHINE high yield capped RNA transcription kit containing T7 RNA polymerase (Ambion).
The OTUD-6B (C188S) mutant was generated by using a QuikChange TM site-directed mutagenesis kit (Stratagene) to replace Cys188 with Ser according to the manufacturer's instructions.
