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The fraction III, containing antimicrobial activity was further subject to CM-Sephadex C-25 cation-exchange column, and nine sub-fractions were collected (Figure S1b).
One microgram of genomic DNA was further subject of bisulphite transformation using the EZ DNA Methylation Gold Kit (Hiss Diagnostics, Germany) in order to be used in pyrosequencing reactions.
The fragmented DNA was further subject to end repair, addition of "A" bases, ligation of NGS adaptors, gel selection of 300 bp fragments and PCR enrichment according to the protocol provided by Yale Center for Genome Analysis (YCGA, http://ycga.yale.edu/sequencing/Illumina/protocols.aspx) with reagents ordered from NEB.
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The second bearing prototype was further subjected to a repeatability test.
The optimized tablet was further subjected to scanning electron microscopy to reveal its release pattern.
The model was further subjected to virtual screening and in-silico docking.
was further subjected to dehydration of acetic acid at varied process conditions, i.e., feed concentration, temperature and downstream pressures.
After acid treatment, the solution was further subjected to electrochemical oxidation and electrochemical Fenton treatments using graphite electrodes.
This compound was further subjected for evaluation of cardiotonic activity (contractile and chronotropic effects) in comparison with Vesnarinone.
Pooled fraction PF1 was further subjected to affinity chromatography.
The fermented broth was further subjected to filtration using Whatman No. 1 filter paper.
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