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The supernatant was further precipitated with 4 volumes of ethanol to recover chondroitin and eventually residual low Mw HA.
DNA was further precipitated with alcohol in order to obtain highly purified DNA.
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DNA was further precipitated 30 minutes with isopropanol at -20°C.
The BAC DNA was further precipitated at room temperature with 0.6 volumes of isopropanol for 30 min and centrifuged at maximum speed for 10 min. The pellet was washed with 70% ethanol, air dried shortly and dissolved in 25 μl of TE.
N3-2A-F/APP complexes were isolated and further precipitated with streptavidin-beads.
Total RNA was further purified with chloroform and precipitated with isopropyl alcohol.
The precipitate was further purified with a silica gel column using ethyl acetate/hexanes (50∶50) as eluent to yield the reddish powder of CRANAD-5 (1.8 g, 52.7 %).
Precipitated DNA was further purified with PCR purification according to manufacturer's instructions (Qiagen).
The immunocomplexes were precipitated once again with protein A agarose beads, washed, and eluted in 100 ml of TE with 0.5% SDS and 200 mg/ml proteinase K. Precipitated DNA was further purified with phenol/chloroform extranction and ethanol before amplifying target DNA by reverse transcriptase-polymerase chain reaction (RT-PCR).
The RNA was further extracted with chloroform-phenol and then alcohol precipitated.
Material precipitated by immunoprecipitation-competent sera was further immunoblotted with anti-gp210 antibodies.
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