An example of the merged alignment in which the hypervariable region was further hand-curated is given in Figure S2d.
After three washes, ovaries were further hand dissected and mounted in VECTASHIELD (Vector Laboratories, Burlingame, CA) for microscopy imaging.
After a tank is gas-free, it may be further hand-cleaned in a manual process known as mucking.
The data sets produced by general filtering were further hand-annotated to reduce the number of probes with multiple annotations.
References which were deemed to be relevant by the authors of this review were further hand-searched, with the useful information extracted for discussion in this paper.
The references listed in the included articles were further hand-searched for other surveys that had not been identified by our database search strategy.
This search was further supplemented by hand searching of relevant references from review articles and other eligible studies.
The automated alignment was further improved by hand to define a seed MSA.
The alignment was further edited by hand to obtain the locus-specific alignments.
The literature search was further enhanced by hand searching 22 journals for the last 5 years (2008 2012).
The resulting fungal material was further purified by hand under a binocular microscope to remove root fragments.
