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Chromatin was further cleared using Protein A and G beads (Sigma, St . Louis MO) and immunoprecipitated with 5 µg anti-Pax6 antibody (H-295X, Santa Cruz Biotechnology, Santa Cruz, CA) in a total volume of 1 ml.
The remaining NM-cell-containing supernatant from the initial CM settling was further cleared of CM cells by sequential centrifugation (4×1 min) at 500 rpm at 4°C until no CM pellet was visible.
In a final QC process, this set of calls was further cleared from variants with an allele balance (variant allele reads/total number of reference plus variant allele reads) below 20% and/or with significant strand bias (i.e. sites at which the variant sequence did not appear on both forward and reverse reads) in spite of high variant quality.
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Samples were further cleared (three times) in Murray clear (1 2 benzyl alcohol:benzyl benzoate).
Cell lysates were cleared by centrifugation (10,000 g; 20 min), and the supernatants were further cleared (100,000 g; 30 min).
Lysates were cleared by centrifugation and supernatants were further cleared by pre-incubating with 25 μl of G-sepharose.
Dundee should have been further clear by the break but Clarkson missed a free header and Twardzik saved well from Paul McGowan.
From the TEM and AFM results, there is further clear evidence of sheet morphology of ZnO with the sheets varying considerably in size, ranging from hundreds of square nanometres to several square microns.
Tevez coolly converted from 12 yards to put them back in front and his side could have been further clear had substitute Fernando Llorente showed more composure over two late chances.
Although Suu Kyi claimed that there haven't been further "clearing operations" since September 5, satellite images of burning villages flatly contradict that claim.
For A. fallax, it is further clear that its demographic response to large-scale hydrological events is not synchronized between the Atlantic and Mediterranean basins.
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