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Finally, the human NIR pathway was fully reconstituted in vitro using the purified APE1, human flap endonuclease 1, DNA polymerase β and DNA ligase I proteins, thus establishing the minimal set of proteins required for a functional NIR pathway in human cells.
The spleens in BM-transplanted eNOS−/− mice expressed high levels of EGFP because their haematopoietic tissue was fully reconstituted by EGFP+ donor cells.
We found that donor tissue from wt and RARα1/KO mice was able to repopulate the recipient glands; the highly branched ductal tree of the RARα1/KO donor was fully reconstituted in a wt-FVB recipient, indicating that the phenotype was epithelial cell autonomous.
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This put sporadic strain on the Roman frontier, until, in the 4th century, the limes at the Rhine and Danube collapsed, never to be fully reconstituted.
To address this problem, we are trying to engineer a novel strain of Saccharomyces cerevisiae in which the cholesterol biosynthetic pathway of mammalian cells has been fully reconstituted.
This indicates that nucleosome presence is necessary for transcription, and temporarily or partly evicted nucleosomes may be fully reconstituted following Pol II passage.
Only mice with >1% engraftment of human CD45+ cells reported to total splenocyte count were considered to be fully reconstituted and were included in the study.
kcat for Glu tRNAGln synthesis is fully reconstituted in this RNP, and Km(Glu) is unchanged, but Km tRNA) is elevated 3-fold (Table 1).
Nevertheless, the human or bovine immune systems can be fully reconstituted in this model using previously reported techniques (Shultz et al., 2007).
We hypothesize that the Mn2+A site must be fully reconstituted with an additional histidine ligand to reintroduce significant metal binding activity, as designed in mutant MA2.
Of note, at post-IR 45 days, when spermatogenesis is supposed to be fully reconstituted, the impregnation rate of D-GHRP-treated mice was still much lower than that of vehicle-treated mice.
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