Sentence examples for was frozen down from inspiring English sources

One of the resulting plates was frozen down and screening of clones was performed by Southern blots on the other plate, following digestion of isolated genomic DNA with BamHI or EcoRV and using "Rosa26-5'", a 502 bp probe, located upstream of the insert and amplified from the Rosa26 locus using the primers 5'-GATAGGAACTGGAAAACCAGAGGA-3' and 5'-GTAAGGGTCCAACAGAAAAGAGA-3' (Fig. 1A).

A sample was frozen down at each serial dilution.

After 30 min of rotation in the cold room and subsequent centrifugation, protein was quantified and 20 µg of protein was frozen down as input controls.

This culture was frozen down in 10% freezing medium consisting of 10% dimethylsulfoxide (DMSO) and 90% fetal calf serum.

All blood samples were centrifuged and serum was frozen down to – 30°~-70°C 30°~-70°Cy were tested for hepatitis B & C markers and biochemical funtilon of theyliver, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyltranspeptidase (GGT).

Equal numbers of cells from each media were pooled together, a fraction of this pool was frozen down to measure the initial frequency of each mutation (starting population) and the remaining cells were selected by FACS for the noted traits twice a day for 5 days.

On the same time we strongly suggest the further use of the same cell lines been transfected in this studies in Orthotopic Xenotransplantation Studies, as we best stable cells were frozen down already.

Aliquots were frozen down and thawed as needed for infections.

Cells were frozen down in multiple aliquots at passage 3 to 7, and stored in liquid nitrogen until use.

When cultures reached confulence cells were frozen down in liquid nitrogen until further use.

Initially, cells were frozen down after every passage, however after passage 20 this was reduced to every five passages.