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Every chromosome of A.gambiae was fragmented, from 5′-end to 3′-end, using a sliding window of 90 nt and a shift increment of 45 nt.
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After treatment with 250 mg/L VD-toxins for 36 hours, DNA from the non-transformed negative control leaves was fragmented, whereas that from the transgenic lines was not.
The mitochondrial DNA was fragmented by nebulization and fragments from 500 to 700 bp were selected for sequencing.
The chromatin obtained was fragmented to sizes ranging from 100 to 300 bp using a Bioruptor (Diagenode, Inc., Seraing, Belgium) for a total of processing time of 140 min (15 s on, 45 s off), with power setting at 'H'.
Poly-A containing mRNA was purified from 2 μg of total RNA, RNA was fragmented, double-stranded cDNA was generated from fragmented RNA and the index containing sample identifier adapters were ligated to the ends.
The biotinylated cRNA from KRSOS was fragmented and hybridized on Agilant Platform microarray (Surechip G3v2).
For 454 sequencing, ~10 ug of genomic DNA was fragmented using a HydroShear apparatus from Genomic Solutions Ltd, and 454 library preparation was done following manufacturer recommended protocols using the Titanium Rapid Library Preparation Kit, with insert sizes greater than 1000 bp.
cDNA from SPIA was fragmented and labeled with biotin, and then it was hybridized using GeneChip® human gene 1.0 ST arrays (Affymetrix) for 18 hours at 45°C with 60 rpm rotation.
Sequencing vectors from these cells identified nine IFP that were fragmented distal from the termini (>25 residues).
Research activities in various health-related fields have been fragmented, isolated from each other, and wastefully duplicative.
Even though some NUPTs escape from the early unstable stage, they are fragmented and eliminated from the genome.
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