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The gradient was fractionated with a density gradient fractionator (Foxy Jr. Fraction Collector, ISCO), whilst monitoring absorbance at 254 nm using a UA-6 UV detector (ISCO).
In this paper, a commercial impact-resistant polypropylene alloy sample was fractionated with preparative temperature rising elution fractionation (t.r.e.f).f
Serum was fractionated with ammonium sulfate, and the fraction precipitating between 50 and 80% of saturation was collected by centrifugation, and dialyzed.
Dried powder of S. cordata whole plant was extracted with methanol and the resultant (SCME) obtained was fractionated with escalating polarity to obtain n-hexane fraction (SCHE), ethyl acetate fraction (SCEE), n-butanol fraction (SCBE) and the remaining soluble portion as aqueous fraction (SCAE).
The sample was fractionated with reversed-phase high performance liquid chromatography (RP-HPLC) collecting thirty fractions at two-minute intervals.
The powder was fractionated with mesh to collect particles at sizes less than 77 μm in diameter.
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To separate nuclear and cytoplasmic fractions, cells were fractionated with NE-PER NuCytoplasmicytoplasmic Extraction Reagent (Pierce Biotechnology, Rockford, IL, USA).
The samples were fractionated with Ciphergen's serum fractionation kit with anion exchange resin in 96-well format into 6 fractions.
To increase the coverage of proteins in SELDI protein profiles, the blood samples were fractionated with HyperD Q (anion ion exchange) into six fractions.
Then, the peptides were fractionated with ultrafiltration membranes.
Natural compounds from flowers, leaves and bark were fractionated with hexane, ethyl acetate and water and bioassayed using either these solvents or a common DMSO-buffer medium for the three fractions.
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