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At the end of the run, the gradient was fractionated from the top into 250 μl fractions.
To accomplish this goal, IgG was fractionated from sera collected from weanling beagles and regressor beagles and tested for conferring protection by passive immunization.
The core OLF product was fractionated from smaller digestion products on a Superdex 75 GL column (GE Healthcare) equilibrated with Buffer A. The core-OLF sample was further analyzed by CD as described above.
Using the newly-developed 1-mm probe, Schlotterbeck et al. [1] were able to acquire 1H13C gHSQC 2D NMR spectra of 1 µg of ibuprofen within 20 hrs, and long-range 1H13C gHMBC spectra of 20 µg of ibuprofen within 5 hrs; they also acquired a 1H-NMR spectrum within 14 min of 1 µg of boldine that was fractionated from a 1-mm HPLC column.
Human endogenous IgG was fractionated from human serum samples of 20 healthy donors using MabSelect (Amersham Biosciences, Piscataway, NJ).
Plasma or serum was fractionated from the blood of 31 patients with metastatic or unresected colorectal cancer and from 28 normal volunteers.
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Gradients were fractionated from the bottom (1 ml fractions) and analyzed for PABP, B5 or ICP4 by Western blotting.
After centrifugation for 18 h at 100,000 g, gradients were fractionated from the top into five fractions of equal volume (with the solubilized pellet as the bottom fraction) and analyzed by SDS-PAGE, using a Tris-Tricine buffer system [65] and phosphorimaging using a Storm 840 phosphorimager and allied software (GE Healthcare Life Sciences, Piscataway, NJ).
The gradients were fractionated from the top manually by pipetting into ∼1 ml fractions.
Four components (P1 P4) with different molecular size were fractionated from the conjugate.
A series of alkanes (C14 C28), phenolic compounds and aromatic nitrogen species were fractionated from H-tar by SGCC and analyzed by gas chromatography mass spectrometry (GC MS).
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