The autotransplantation of inferior bulbar conjunctiva to sclera bare area on nasal side was for minimal excision of conjunctival tissues to save conjunctival goblet cells as much as possible, which could prevent further decrease of mucoprotein in tear fluid.
In the presence of plasmid pSf2032 carrying an integrase-defective class 2 integron (whose attI2 sequences were shown to be recognized by IntI1), the recombinant integrase was shown to be active for excision activity (40% of cassettes lost).
Multiple treatments following 'failed DAIR' cost on average $29,560, for failed 1-stage revision were $24,357, for failed 2-stage revision were $70,381 and were $23,805 for excision arthroplasty.
In 22 procedures, a minisnare was used, and in 6 cases a prototype snare was designed for excision of the papilla.
They have been shown to be effective for excision of unwanted DNA from the genome of the host but require complex schemes for integration.
One interpretation of this reduced activity is that the lines were undergoing low levels of Bxb1-mediated excision, but PCR analysis of lines where GUS activity was weak or undetectable were positive for excision of the target DNA.
In this case, it was demonstrated that a factor called Hef RDF, which is encoded by HPI, is required for excision (Lesic et al., 2004).
Initially we attributed this reduced activity to lower levels of phiC31-mediated excision, but PCR analysis of lines where GUS activity was weak or undetectable were positive for excision of the target DNA.
Based on the lowest EGFP expression level, one parental line was chosen for excision.
The exon' 4 of the basonuclin gene, which encodes the critical zinc fingers and the nuclear localization signal, was targeted for excision by the Cre recombinase.
