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The other half of each brood was first surface sterilized in an antimicrobial solution of hen egg-white lysozyme (1 mg/ml), streptomycin (500 μg/ml) and ampicillin (100 μg/ml), and then placed onto water agar plates to hatch.
Uniform sized MSN was first surface modified with amino groups, followed by chelator (e.g., S-2- 4-isothiocyanatobenzyl -1,4,7-triazacyclononane-1,4,7-triacetic S-2- 4-isothiocyanatobenzyl -1,4,7-triazacyclononane-1,4,7-triacetictope S-2- 4-isothiocyanatobenzyl -1,4,7-triazacyclononane-1,4,7-triacetic
In order to impart hydrophilicity on these UCNPs, they were first surface modified prior to be used.
Solid samples (salt rock) were first surface-sterilized by immersing 200 g of the sample in ethanol followed by flaming.
It's just that the subprime sector, being one of the most vulnerable spots, is where the problems are first surfacing.
HNTs were first surface treated by methyl, tallow, bis-2-hydroxyethyl, quaternary ammonium, then melt mixed with PP.
Stromata and sclerotia were first surface sterilized with the same procedures as above, and then genomic DNA was extracted with the traditional CTAB method [36].
There is one report of intracellular staining of LIN− cells that were first surface-stained for Sca-1 and Kit, and then fixed and saponin-permeabilized with a commercial kit [4].
To determine the intracellular expression of the Treg cell specific transcription factor Foxp3 in CD4+CD25+ cells, mouse splenocytes were first surface-stained with PE-labeled anti-mouse CD4 (RM4-5) and APC-labeled anti-mouse CD25 (PC61.5) from eBioscience.
