Sentence examples for was first stimulated from inspiring English sources

TRPM3 was first stimulated by a high concentration of nifedipine (Wagner et al., 2008), leading to Ca2+ influx.

The inhibitory effect of BD1047 was confirmed in whole-cell patch clamp recordings in which TRPM3 was first stimulated by an alternative TRPM3 agonist PregS.

The muscle is first stimulated to contract isometrically at its optimal length.

Differentiated HL-60 cells were first stimulated with a low dose of fMLP before we triggered iRap induced Rac activation.

Thus, tolerant T cells were first stimulated with peptide and IL-2 for 7 days, washed and re-stimulated with peptide in the absence or presence of IL-2.

THP-1 monocytic cells were first stimulated with increasing amounts of M. tuberculosis-sonicate antigens or human rMCP-1 and expression of MMP-1 was monitored by real-time PCR.

CHO cells expressing truncated and mutant forms of SERT were first stimulated with 100 μM 5HT, and then biotinylation assay was performed to separate the plasma membrane proteins and their partners.

Adherent cells were cultured in 96-well plates and 48 hours after transfection cells were first stimulated with AVP in DMEM-serum free supplemented by BSA 0.1% and then washed with twice PBS.

Platelets in platelet poor plasma (PRP) were first stimulated with 1 or 2 nM 5HT, which represents the plasma levels of 5HT in normotensive and hypertensive patients, respectively, and thus a physiologically relevant stimulus [16].

For the analysis of cytokine production by intracellular staining cells were first stimulated with PMA (Sigma-Aldrich) and ionomycin (Sigma-Aldrich) for 4 h at 37°C in IMDM medium plus 7% FCS.

In other experiments, CD4+CD25+ T cells were first stimulated with CD3 antibody alone (0.5 µg/ml) or in combination with anti-GITR antibody (50 µg/ml) for 48 hrs and were then co-cultured with freshly isolated CD4+CD25− T cells in the presence of APCs and CD3 antibody.