For fluorescence imaging with RNase A@C-dots, MGC-803 cells were first plated on 14-mm glass coverslips and allowed to adhere for 24 h at 37°C.
Bone marrow cells were first plated onto tissue culture plastic (30 min, 37°C) and the adherent (macrophage-rich) cells discarded.
For microscopy, cells were generally plated on coverslips in 24 well dishes after nucleofection or were first plated and then transfected using Fugene.
For single-cell cloning experiments, rat CDCs were first plated at an assumed density of 1 cell per well in a 96-well plate coated with fibronectin.
Colonies were first plated on LB/Tet/IPTG to select for nadB::Tn10 and to overexpress the putative suppressor gene on the plasmid.
To examine the effects of reoxygenation, cells were first plated at 50 cells/cm2 and incubated under either normoxic or hypoxic conditions for 8 days.
In order to determine the role of paracrine signaling between fibroblasts and carcinoma cells the assay was modified so that fibroblasts (2.5×104 fibroblasts / ml) were first plated in wells as a monolayer and incubated for 24 hours.
For all experiments, HepG2 cells were first plated on to collagen-coated plates.
Cells were first plated on bacterial (non-coated) plates for 2 days and then transferred to standard cell culture plates for additional 5 6 days before stimulation.
Transformants were first plated on selective agar plates without uracil, histidine, tryptophan and leucine, and with maltose as a permissive carbon source.
