Sentence examples for was first maintained from inspiring English sources

To establish an androgen-independent cell line, LNCaP was first maintained in phenol red-free DMEM/F-12 medium (Gibco) with 10% charcoal/dextran-treated FBS (Biowest).

P. stipitis Y7 strain was first maintained and cultured by sequentially transferring and growing cells in a medium containing low concentrations(10 mM/L) of furfural supplemented with 10 g/L yeast extract, 2 g/L peptone, 20 g/L glucose.

The samples were first maintained at 260 °C for 320 s, following the conventional reflow-soldering methodology.

The fungal cultures were first maintained separately as slants in culture tubes containing Potato dextrose agar (PDA) medium.

When water enters at the toe of the wellbore, its holdup is first maintained at 1 because there is no oil production during that time.

Subjects were first maintained in dim light (<5 lux) for 2h and then scanned during three consecutive 20 min sessions (Figure 1a).

Mice were first maintained at a barrier facility in the Department of Molecular and Cellular Biology at Harvard University under animal protocol 93-15 and later maintained at a barrier facility at the Centre for Regenerative Medicine, Massachusetts General Hospital, Harvard University under animal protocols 2006N000104 and 2009N000050.

Transfected parasites were first maintained for a period of 1 2 weeks in Swiss mice under pyrimethamine drug pressure.

The monolayers were first maintained under open-circuit conditions while the transepithelial potential difference (Vt) and resistance (Rt) were monitored and observed to reach a stable level.

The seedlings were first maintained under a 12-h light/12-h dark photoperiod (12L/12D) for 3 days, and then under relatively long-day light conditions (16L/8D), relatively short-day conditions (8L/16D), or continuous darkness for 3 days (DD).

To evaluate segregation and maintenance of the PCEN-containing constructs during mosquito development, transfected parasites were first maintained for a period of 1 week in Swiss mice under pyrimethamine drug pressure.