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The manure was first inoculated with S. enterica serovar Typhimurium, S. enterica serovar Enteritidis or enterica serovar S. Dublin (108 CFUs per g fresh weight) and mixed thoroughly before addition to soil at a ratio of 1 10 fresh weight.
For each experiment, a column was packed with porous medium that was first inoculated with bacteria that contained the NAH plasmid encoding genes for the degradation of naphthalene and salicylate, and then subjected to a step input of salicylate solution.
For identification, fungus was first inoculated in PDB (Potato dextrose broth) and incubated at 28 °C till growth appeared.
The yeast mixture was first inoculated into the selection medium with newspaper as a sole carbon source.
Aspergillus niger stored in the slants was first inoculated to plate medium and cultivated at 35 °C for 60 h, then fungal colonies on the plates were selected randomly, inoculated to 250 mL eggplant bottle slant with 50 mL medium and cultured at 35 °C for 48 h until the slant covered with dense spores.
Virus-containing brain homogenates were obtained as follows: 0.02 ml of each viral suspension was first inoculated intracerebrally into newborn mice and the animals were observed daily.
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Cell layers were first inoculated with Chlamydiaceae and 20 h later with ca-PEDV in protocol one.
Babies may be first inoculated against whooping cough at birth rather than at two months because of a burgeoning epidemic that has claimed the lives of six infants this year.
Since these four crops commonly harbor multiple viroid species, including HpSVd, the LMW-RNAs were first inoculated into cucumber (Cucumis sativus, cv.
Mice were first inoculated via the right tail vein with 8ng of RvE1 per gram of mouse or vehicle (<0.1% ethanol) diluted in sterile PBS.
To more closely model natural human exposure, co-infected animals were first inoculated with P. cynomolgi and then with SIV following the third parasite relapse for a total of four parasitemias (Figure 1).
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