To ensure that the lens was not damaged during dissection, each lens was first incubated for 4 hrs in a humidified incubator under 95% air and 5% CO2 at 37°C and checked for clarity [32].
In this work, the library was first incubated with PLAP and the bound clones then eluted by incubation with L-Phe-Gly-Gly along with the substrate, para-nitro phenyl phosphate (pNPP).
Project Loon was first incubated by the experimental Google X division in 2011, but was officially announced in June 2013 with a remit to help bring the internet to the two thirds of the world's population who still don't have access.
To date, Buffer, which was first incubated in AngelPad, has raised some $400,000.
Other investors in the round include previous backers True Ventures (where MessageMe was first incubated), First Round Capital, Google Ventures, SVAngel, Resolut.vc, Andreessen Horowitz, and Social+Capital Partnership.
The site, founded in 2004 by Matt Rutledge when it was first incubated inside Rutledge's preexisting company Synapse Micro, was one of the pioneers of the "daily deal" model of selling goods online.
The company, originally launched to the web in 2009, was first incubated by [212]Media, a company-building firm which both invests and develops startups, leveraging their close ties to nearby New York media companies.
Prajapati, the creator of all living things in Indian mythology, was born of a great golden egg, which was first incubated in the uterine waters of eternity.
IP5 was first incubated with IP6K1 and Vip1 was subsequently added to the assay.
The reaction was first incubated at room temperature for 2 h.
The membrane was first incubated with the anti-DNP rabbit polyclonal antibody (1∶150), then stripped and subsequently reprobed for anti-α-tubulin and anti-Tom20 antibodies.
