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In brief, a standard curve was first generated to show that the immunoreactive LTKE-FITC peptide in solution can compete for HRP-conjugated FITC antibody (Jackson Laboratories, West Grove, PA, USA) binding with solid phase FITC.
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Inspired by the random colloid-aggregation model which explains the formation mechanism of random porous media, Voronoi tessellation is first generated to partition the space into a collection of compartments.
For example, the CCB, as a policy proposal, was first generated due to the advocacy of community stakeholders, including invested government officials like Senator Sharon Carstairs who, in 2000, published her report Quality End of Life Care: The Right of Every Canada.
A simple job and process profile was first generated for all facilities to allow an overall understanding of the prevalence of heat exposure situations in companies.
For gene ontology analysis, ranked gene lists were first generated according to the moderated t statistics from linear models and then examined for enriched ontology terms using the Gene Set Enrichment Analysis software [ 15].
To ensure in-frame alignment, amino acid sequence alignments were first generated and then used to guide the nucleic acid sequence alignments.
Spectrally equivalent ground motions were first generated from historical records to decouple the effect of duration from those caused by other characteristics of ground motions.
The network contains also other potential alternative mechanisms for the complete oxidation of methanol in which multi-carbon compounds are first generated and then completely oxidized to CO2.
When no Mo is contained, tiny clusters of Bi2Se3 nanosheets are first generated upon heating and then enriched to assemble into bigger nanosheets.
As a proof of principle the Agent was first trained to generate molecules which do not contain sulphur.
Production of shale oil began in 1921 and oil shale was first used to generate electrical power in 1924.
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