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A homology model of AgAChE was first generated by the SWISS-MODEL program {http://swissmodel.expasy.org//SWISS-MODEL.html [7]} according to multiple sequence alignments using X-ray structures of two mouse and one electric eel AChEs as templates (Figure 1).

A calibration factor for converting units of microPET images into absolute radioactivity concentration units was first generated by imaging a phantom filled with a known concentration of FDG.

The selective marker ura4 + (InvU4) was first generated by PCR with primers InvU4S and InvU4-AS and wild type KRP1 genomic DNA as the template.

Tc was defined as the period between when a cell was first generated by cytokinesis and when that cell subsequently divided, giving birth to two daughter cells.

A calibration factor for converting units of microPET images into absolute radioactivity concentration units was first generated by imaging a phantom filled with a known concentration of [F]FDG.

For positive control normalization, a normalization reference was first generated by calculating the sum of the counts for the positive spike in transcripts in each sample and then calculating the average of these sums across all samples.

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The product platform of a specific product is first generated by designers or domain experts using general sorting, a requirement acquisition technique, and design knowledge hierarchy (DKH), a knowledge representation structure.

The 4-D structures are first generated by 3-D printers but then transform when activated.

The training data are first generated by calculating FEGT using heat balances through various heat exchangers.

In the proposed scheme, master shares are first generated by extracting features from temporally informative representative images (TIRIs) of both the 2D video frames and depth maps.

All of these methods begin with a standard preparative method where H2WO4 is first generated by passing a Na2WO4 solution through a cation-exchange resin.

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