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Mouse cardiac tissue was first fixed on Bouin's fixative and stained with Weigert's hematoxylin and Biebrich scarlet-acid fuchsin (Sigma, Milwaukee, USA), followed by treatment with phosphomolybdic phosphotungstic acid solution (Sigma) and staining by methyl blue and Mayer's Hematoxylin and Eosin.
The nerve segment was first fixed for a few seconds in a small drop of fixative solution (glutaraldehyde 2.5%and0.5%5% sucrose in 0.1 M Sörensen phosphate buffer, pH 7.4) to maintain the nerve straight in order to sustain specimen's orientation; then, the specimen was placed in an Eppendorf tube with the fixative solution for 6 8 hours.
During this stage, the backbone of the protein was first fixed and then harmonically restrained.
The volume of granulation liquid was first fixed at the same level whatever the granulation equipment and the formulation.
Subsection (b) of section 109 deals with the scope of the copyright owner's exclusive right to control the public display of a particular "copy" of a work (including the original or prototype copy in which the work was first fixed).
Ms. Breloh of the museum says the dish was first fixed in 1949 by Herta Heuwer, a Berliner, who like many others in postwar Germany struggled to make ends meet.
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Upon addition of EDC fixative embryos were first fixed for 2 hours at RT followed by o/n fixation at 4°C, while on a 3D rocker.
The term "phonorecords" includes the material object in which the sounds are first fixed.
The term "copies" includes the material object, other than a phonorecord, in which the work is first fixed.
After 2 3 weeks, the cells were first fixed by 4% paraformaldehyde and then stained with 0.05% crystal violet.
For intracellular staining, cells were first fixed in 4% paraformaldehyde and then surface stained with CD14 and CD3.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com