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To prepare the DNA for MinION sequencing, the DNA was first end-repaired and then dA tailed using NEBNext End-Repair, and NEBNext dA-Tailing Modules (New England BioLabs) according to manufacturer's instructions.
The V3 loop amplicons were first end-repaired with T4 DNA polymerase and 5'-phosphorylated with T4 Polynucleotide Kinase (454/Roche).
The recovered DNA was first 5' and 3' end blunting, phosphorylating and repairing by T4 Polynucleotide Kinase and T4 DNA Polymerase (NEB).
To ensure sufficient separation between distinct compositions, an excessive splitting was first performed, ending up with 32 clusters.
A DNA sequence with –SH at one end was first immobilized on Au electrode.
In Mainland China, a poultry vaccine was first used at the end of 2004.
Poly methyl methacrylate) (PMMA) with a halide end group was first prepared by ATRP of MMA.
A seasonal ice boom was first installed across the eastern end of Lake Erie in 1964-65.
All offspring were first single end sequenced at 80 bp on the GAII.
"I was fourth at the end of the race, with Jenson to pass".
For data analysis, low quality samples and non-expressed miRNAs were first removed ending up with 29 individuals from 9 families and with 333 miRNAs.
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