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Applying a transformation technique, the effect of auto-correlation was first eliminated and then, each of the three parameters of a simple linear profile was monitored separately by an EWMA control chart until a signal was generated.
pYES2-s-Fz2-myc: In order to be able to replace the Fz1 HpaI-EcoRI fragment from pYES2-s-Fz1-myc with the respective Fz2 fragment, the second HpaI site within the pYES2 vector backbone was first eliminated using GeneTailorTM (Invitrogen) site-directed mutagenesis kit with primers DD_36 (5'-CACTTCAATAGCATATCTTTGTAAACGAAGCATCTG-3') and DD_37 (5'-AAAGATATGCTATTGAAGTGCAAGATGGAAAC-3').
To examine the effect of introducing a second deletion mutation, the kanamycin resistance gene was first eliminated from deletion mutants through FLP recombination mediated by pCP20.
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"The nuance in a turnaround is that sometimes jobs are first eliminated during a restructuring, often a necessary step before new jobs and growth can be created".
To retrieve significant information recorded by the TBA, DC-offset is first eliminated by subtracting the mean amplitude from raw data.
To reduce computation demands in calculating the seismic response variation, design parameters that have a negligible effect on seismic response are first eliminated through sensitivity analysis.
In this case, probe sets that were not expressed in WAT were first eliminated, leaving a remainder of 22,007.
Lineage+ and dead cells were first eliminated by flow cytometric sorting.
To investigate population structure, close markers that were in LD were first eliminated because these might cause a bias in the estimation of population structure.
The sensible thing is for the bees to be first eliminated by dusting with Permethrin powder propelled at high pressure.
Strong was the first eliminated after being pinned by Aries following a 450° aerial splash.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com