Sentence examples for was first dissociated from inspiring English sources

Tissue was first dissociated in 0.25% trypsin (Invitrogen, Burlington, Canada) and 25 μg/mL DNase I (Roche, Laval, Canada) for 30 min at 37°C and then passed through a 140 μm nylon mesh.

For FACS analysis, cells were first dissociated with 0.05% Trypsin in 0.2% EDTA and PBS.

For electroporation, ES cells were first dissociated by trypsin into single cells.

Cells were first dissociated into single cells by trypsin and seeded into 96-well plate (1 × 10 cells per well).

Ex vivo resected treated 9L tumours were first dissociated in DNAse/collagenase solution and recultured before cytochalasin-B was added.

For oligos used, see Table 4. ChIP was performed on nuclei derived from induced or transiently transfected ES cells (see above) or from seminiferous tubules in which multiple testicular cell populations were first dissociated by enzymatic digestion of seminiferous tubules and subsequently isolated by elutriation.

In the light scattering experiments described below ribosomes were first dissociated into their subunits in the presence of mRNA and initiation factors IF1 and IF3 in a buffer of indicated composition and then initiation factor IF2 GTP was added together with fMet-tRNA to dissociated 70S ribosomes in the stopped-flow instrument.

For enzymatic dissociation, tissue was first minced into ∼1 mm3 pieces and then incubated on a stir plate at room temperature until tissue was dissociated (1 18 hours).

Samples were first thoroughly minced and then enzymatically dissociated by a 6 h digestion at 37°C with collagenase II (Roche Diagnostic, Mannheim, Germany) 0.05 mg ml−1 in DMEM : F12.

It is to be noted that the formation of semiclathrate hydrate is achieved first and then is dissociated to reach up to equilibrium point B as in Figure 6.

Flow cytometry of whole embryos (pools of 5 embryos, > 10 biological replicates) was performed by first dissociating embryos enzymatically using Liberase 50 μg/ml (Roche) at 37°C for 2 hr followed by mechanical dissociation by pipetting.