In order to determine whether Twist can bind to the myogenin promoter and therefore contribute to the mechanism of reversal of differentiation, C2C12 muscle cells were first differentiated in vitro to multinucleated myotubes and then transduced with an AdT.
In order to correctly code the reform, the overall reform package has been first differentiated by policy subdomain and then within each subdomain, every change has been identified separately.
The stem cells are first differentiated into endodermal cells (D'Amour et al., 2005), subsequently into hepatoblasts, and finally into hepatocytes, mimicking liver developmental processes.
To prepare macrophage-conditioned (MC) medium, THP-1 monocytes were first differentiated by the addition of 100 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma) for 48 h.
To study the role of distinct macrophage populations on the modulation of cancer cell-related activities, primary human monocytes were first differentiated into macrophages, and left unstimulated or stimulated with 10 ng/ml of LPS (LPS-stimulated) or IL-10 (IL-10-stimulated), respectively.
In this procedure, stem cells were first differentiated into a novel type of cells harbouring the post-implantation epiblast status, called epiblast-like cells (EpiLCs); after induction with a cocktail of other growth factors, EpiLCs robustly (about 30%) differentiated into PGC-LCs resembling their in vivo counterparts.
The isolates were confirmed as B. bovis by using gltA-PCR assay followed by sequence analysis.> -wrap-foot> Malele, F Female, YKK Yellow cattle cross Kedah Kelantan The isolates were first differentiated based on the nucleotide variation of gltA, ITS, rpoB and ERIC-PCR assay (Table 2).
Snrk was first identified in 3T3-L1 differentiadipocyteocellscells (Becker et al., 1996), and subsequently was shown to be expressed in a host of other tissues in mice and humans including white and brown adipose tissue (WAT and BAT), small intestine, heart, brain and liver (Li et al., 2013), tissues with high metabolic needs.
The number of DSBs occurring after treatment in either pre-adipocytes or differentiated adipocytes was first measured, using the phosphorylation of H2AX, a variant form of histone H2A [29].
Rat pheochromocytoma PC12 cells were first fully differentiated to neuronal-like cells by nerve growth factor (NGF) treatment, mimicking the terminally differentiated neurons in adult brains [ 16].
