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Silica gel column was first conditioned with Milli-Q water followed by the eluting solution, and then Fr-DS-3 was loaded to obtain fractions (25 mL each).
The fibre was first conditioned for 30 min at 230°C in the injection port of the gas chromatograph.
The C-18 SPE was first conditioned using 2 mL of MeOH, 2 mL of H2O and 1 ml of 0.1% TFA.
The column was first conditioned with 30 mL of methanol followed by 30 mL of distilled water.
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The collected data are first conditioned using precision electronic circuits and then interfaced to a PC using RS232 connection via wireless unit.
The collected data are first conditioned using precision electronic circuits and then interfaced to a PC using a data-acquisition card.
The cleaned grains for milling are first conditioned, after which barley is washed-out with diluted sulfur dioxide.
Models were first conditioned on one predictor and the clinical pathological variables, and then the significance of the other was tested.
Slides were first conditioned for 12 h at 48°C in a solution of 5x SSC, 100 ng/μl salmon sperm ssDNA, 5x Denhardt's solution and 0.1% SDS).
In that numerical learning test, individuals were first conditioned to associate a certain number of symbols (2 or 4) with a food reward.
Solid-phase extraction was performed with Sampli-Q OPT cartridges (3 mL, 30 mg; Agilent Inc., Santa Clara, CA), which were first conditioned with 3 mL methanol (MeOH) and rinsed with 3 mL water.
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