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To combine the concentration step with the detection step, M13 was first concentrated following the same protocol as mentioned previously in Partitioning and Concentrating M13.
Smith's new Inland Customs Line was first concentrated between Agra and Delhi and consisted of a series of customs posts at one mile intervals, linked by a raised path with gateways (known as "chokis") to allow people to cross the line every four miles.
Conditioned media (CM) was first concentrated for 30 min at 3000 rpm using Amicon Ulita-5 centrifugal filter device (Millipore, Billerica US) as described by the manufacturer.
After loading, the peptide mixture (7 µl in 0.5% TFA) was first concentrated and washed (i) at 1 µl/min onto a C18 reverse-phase pre-column (Waters) or (ii) at 4 µl/min in 40 nl enrichment column (Agilent Technologies chip), with 0.1% formic acid as the eluent.
Each ethanol filtrate was first concentrated using a rotary evaporator.
The crude enzyme extract was first concentrated by ammonium sulphate precipitation.
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Fractions with low levels of DKC1 were first concentrated using a Spin-X UF concentrator (Corning, Tewksbury, MA) before they were used for immune-affinity purification.
The system consists of two units: particles (including their semi-volatile component) are first concentrated by means of the Versatile Aerosol Concentration Enrichment System (VACES), and subsequently drawn through a heating section, in which semi-volatile particle-bound components partition to the gas phase, while non-volatile particles are removed by a quartz filter placed after the heater.
As a result, the maximum theoretical efficiency of a conventional solar cell is 30percentt, or 41percentt if the sunlight is first concentrated using a mirror or lens.
Under the developed methodology, NO is first concentrated in the microcage of the zeolite structure and the adsorbed NO is subsequently decomposed by efficient energy irradiation of MWs without desorption of NO as intact molecules.
The deformation of the DYB is first concentrated at the small core plate and then shifted to the large core plate once the stopper mechanism is triggered, resulting in double-stage yield behavior of the DYB.
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