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The POPC membrane built using the membrane builder plugin in VMD was first aligned by moving its center of mass to the origin.
To ensure repeatable positioning, the phantom was first aligned with camera's lasers.
The length variable 28S data was first aligned using default parameters in ClustalX v 1.83 [39], then manually adjusted.
Following the pre-processing the functional scan was first aligned to the high resolution EPI scan, then to the high resolution T1-weighted image, which was subsequently registered to the MNI152 standard space (average T1 brain image constructed from 152 normal subjects at Montreal Neurological Institute) using affine linear registration as implemented in FLIRT [41].
For the DTI analysis, each participant's FA data was first aligned into MNI 152 standard space using FNIRT.
A UStag was first aligned within the protein sequence using observed precursor and first fragment mass, preferring tryptic cleavages where possible.
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Input short reads are first aligned with a gapped NGS aligner.
Selected sequences were first aligned with MUSCLE multiple sequence alignment [ 57] and then refined using Gblocks [ 58].
Protein sequences were first aligned using ClustalW2 (Clustal 2.1 multiple sequence alignment, Conway Institute UCD, Dublin, Ireland) for each group of genes.
In many cases, the RNA-Seq reads are first aligned to a reference genome using an alignment tool that identifies possible read origins within the genome.
Sequences were first aligned with the ClustalW program, and the non-aligned segments and gaps were cut with ad hoc perl scripts.
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