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The residue was finally reconstituted with 200 μL of the desorbing solvent.
The ethanol solution was finally reconstituted with saline solution (9 mL).
MGM was finally reconstituted with new owners.
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After a 10 min centrifugation, pellets were finally reconstituted in 500 μl of 0.1 M NaOH and transferred into scintillation vials.
During the reconstruction phase, normalized data is first restored and a scaling procedure which is exactly the inverse to the normalization is applied to finally reconstitute the data in its original form.
After irradiation, the radioactive foil was dissolved with 0.4 mL of freshly prepared aqua regia (HCl/HNO3 = 3/1, v/v) under heating at 90 °C for 5 min. The solution was then dried by heating at 100 °C, reconstituted in 0.4 mL of 0.05 M HCl twice, and finally reconstituted in 0.1 M HCl for shipping.
Digests were cleaned using OMIX C18 SPE cartridges (Agilent, Palo Alto, CA) using the manufacturer's protocol and eluted in 25 µl of 60/40/0.1% ACN/H2O/TFA, dried to completion in the speed-vac, and finally reconstituted in 100 µL of 0.1% formic acid.
Finally, cDNA was reconstituted in 50 μL DEPC water and frozen at -20°C.
Finally, the residue was reconstituted in isooctane (1 mL) and stored in an amber vial.
Finally, the extract was reconstituted with 1 mL of methanol before HPLC analysis.
Finally samples were reconstituted in 50%H2O-50H2O-50N (v/v) and passed through Millipore Millex-LG syringe filters (filter diameter 4 mm, pore size 0.22 μm) before direct infusion into the mass spectrometer to perform MSn experiments.
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