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Sarkosyl-insoluble material was finally extracted in urea buffer and saved as the urea fraction.
From every production simulation, one structure per nanosecond was finally extracted and its secondary structure content assigned using STRIDE as implemented in VMD's timeline tool (Humphrey et al, 1996).
The pellet from 4 M urea wash was finally extracted in a Polytron homogenizer with 10 mM Tris buffer pH 7.2, 1 mM EDTA containing 8 mol/L urea (extraction buffer).
After washing the DNA plugs, the genomic DNA was electro-eluted (100 V for 1 hour at 4°C) into a dialysis tube with 1X TAE buffer, and the HMW genomic DNA was finally extracted with phenol/chloroform/isoamyl alcohol, precipitated with isopropanol, and resuspended in TE buffer.
The complexes were washed and dissociated from the beads by incubation in 1% SDS in TE and nuclear lysis buffer at 65°C for 10 min. Histones were then digested with proteinase K for 1 h at 45°C and the DNA was finally extracted with phenol/chloroform/isoamyl alcohol and ethanol precipitation.
Similar(55)
FAMEs were finally extracted with hexane.
The man is finally extracted; the peacekeepers are dragging him toward their base down the road.
A polygonal mesh is finally extracted, which is used for visualisation of the bone deformity.
The controller is finally extracted as the solntion to a system of Hamilton-Jacobi equations.
The repeating patterns are finally extracted using a soft masking approach.
In each frequency-band, the number of modes is evaluated, the frequencies and damping factors are estimated, and the complex amplitudes are finally extracted.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com