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The library was finally concentrated to approximately 200-fold after four rounds of panning.
Generally, every 10 ml of supernatant was finally concentrated into 50 μl as one aliquot.
The protein was finally concentrated to 10 mg/mL in a Centriprep-50 microfiltration device, frozen as 30 μL droplets in liquid nitrogen, and stored at −80 °C.
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The Molten Carbonate Fuel Cell (MCFC) can be used as concentrator of CO2, due to the chemical reactions that occurs in the cell stack: carbon dioxide entering into the cathode side is transported to the anode side via CO3= ions and is finally concentrated in the anodic exhaust.
Ascorbate is transported into the posterior chamber by the ciliary body epithelium, enters the aqueous humor, diffuses through the corneal endothelium into the stroma and is finally concentrated by the epithelial cells [62], [63].
The labeled proteins were finally concentrated at 34 uM and 130 uM, respectively.
The organic phases were finally concentrated to dryness under a N2 flow in a Turbo-Vap unit and stored at −10°C.
CMs from CSps (n = 20) and from CDCs (n = 20) were separately pooled and assayed by time-resolved immunofluorometric assay (TRIFA) before diafiltration on X-100A nonionic membranes (Diaflo, 43 mm diameter, Amicon) using a stirred cell (Model 52, Amicon); these CMs were finally concentrated to small volumes with 0.02 M Tris buffer solution, pH 7.2, 0.15 M NaCl.
The Iranian team was finally able to concentrate on soccer in the six-team tournament.
It was concentrated to a dark green residue which was finally dried in vacuum (yield 100 g).
Between each step, the supernatants were pooled, and finally concentrated by evaporation using a centrifugal evaporator (SpeedVac).
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