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RNA from each of the collected samples was extracted utilizing NucleoSpin® miRNA kit (Macherey-Nagel, Duren, Germany) following manufacturer's protocol and recommendations.

Total RNA was extracted utilizing AllPrep DNA/RNA Micro Kit (Qiagen) and reverse-transcribed with the MessageAmp™ II aRNA Amplification Kit (Ambion) to generate Cy3-and Cy5-labeled (Ambion) cDNA probes for adherent and nonadherent samples, respectively.

The samples were extracted utilizing a pressurized fluid extraction (Accelerated Solvent Extractor, Dionex ASE-300) with a mixture of acetone and hexane (1 1, v/v).

Assay samples were extracted utilizing acetonitrile.

For a set of given mode-isolated signals, the un-damped mode shapes are extracted utilizing the singular value decomposition of the output energy correlation matrix with respect to sensor locations.

The system collects data about lane following, vehicle following, speed and engine speed patterns which are important for aggressive driving detection since aggressive driving behaviour is associated with sudden lane changes, tailgating and abrupt acceleration/deceleration. Features that are extracted utilizing these data are used to train an SVM classifier.

Each scattering image features were extracted utilizing 2-D Zernike moment invariants [ 21, 22].

Total RNA from RINm5f, RINm5f-GPR119 and MIN6c4 were extracted utilizing the Ultraspec RNA isolation kit (Biotecx, Houston, TX, USA).

First, matches for venom protein classes identified by InterProScan in BLAST2GO were extracted utilizing a Perl script.

PM signal values for probesets were extracted utilizing raw CEL files matched with probe identifiers obtained from a CDF file (MoGene-1_0-st-v1,r3.cdf obtained from http://www.aroma-project.org/vignettes/GeneSTArrayAnalysis) implemented by Aroma Affymetrix statistical packages run in the R-studio environment (version 0.97.551, R-studio Inc., running with R 3.01).

Perfect Match (PM) signal values for probesets were extracted utilizing raw CEL files matched with probe identifiers obtained from the Affymetrix provided CDF file HG-U133_Plus_2.cdf HG-U133_Plus_2.cdfroma Affymetrimplementedcal packages run in R-studio environment (version 0.97.551, R-studio Inc., running with R 3.01).