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Then, the serum was extracted out and a primary antibody against RAGE (1 : 200) was added to incubate the sections 4 °C overnight.
Repetition of the LLE showed that after two extractions, over 99.9% of the phthalate was extracted out of the sample (Figure 3).
A gel slice containing 200∼250bp PCR products was cut and shredded, and the amplified library was extracted out of the gel by passive elution in elution buffer (1.5M ammonium acetate in 1× TE).
The protein was extracted out of inclusions using guanidine, isolated using Ni-NTA resin (Qiagen, Inc).
Genomic DNA was extracted out of the cryopreserved sera using High Pure PCR Template Preparation Kit HPPTPP kit) according to the manufacturer's instructions (Roche Molecular Biochemicals, Mannheim, Germany).
In addition total RNA for qPCR analysis was extracted out of different individuals than the once used for cDNA library construction and patterns appear to be consistent between the different samples for all the selected candidate genes, which reflect the robustness of the approach and the small, if any bias, contributed by individual outliers.
Similar(54)
The light in the enhanced optical modes will be extracted out by PhC.
By using the SVD updating terms and documents, the true and spurious eigensolutions can be extracted out respectively.
This element is defined to control the order in which packets are extracted out from its queues.
The reaction takes place in a solid liquid liquid mode and most of the cyclohexanol is extracted out by the organic phase during the course of the reaction.
(6) The process in step 5 is repeated on the other elements in the integer set of user until subcarriers have been extracted out for user to transmit.
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