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DNA was extracted individually using the chelex extraction method (Ingram and Gordon 2003) and stored at −20°C.
Total genomic DNA was extracted individually from 10 male nematode samples representing O. dentatum, and 4 male nematode samples representing O. quadrispinulatum.
Genomic DNA was extracted individually from seven sctt and five wild-type flies using a buffer containing 10 mM Tris-HCl, 1 mM EDTA, 25 mM NaCl, and 10 mg/ml Proteinase K [53].
For intraspecific comparison, genome DNA was extracted individually.
DNA was extracted individually from 10 Caullerya-infected Daphnia hosts per lake (Table 1).
Total RNA was extracted individually from each of these samples as previously described.
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Nymphal ticks were extracted individually, whereas same-species larvae from the same individual animal were pooled.
DNA from larvae were extracted individually in 200ul 96-well PCR plates using 25 ul of extraction buffer comprised of 0.5% tween, TE buffer (10 mm Tris, 1 mM EDTA), and 2.5 ul 20 mg/ml Proteinase k (Bioline).
Pulses were extracted individually from the data and assimilated using the raw coordinates.
In addition, 8 plaster samples were extracted individually from western wall of the transit hall, western and northern wall of the main hall, containing two coarse layers and a fine coat.
In the surgery to place the implants, equally to previous surgery, the teeth of the right hemimandibles were sectioned in a bucco-lingual direction using a tungsten carbide bur so that the roots could be extracted individually without damaging the remaining bony walls.
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