Site-directed mutagenesis was carried out by means of PCR with the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene Products Division, La Jolla, CA, USA).. Freshly transformed E. coli Topp3 cells were induced with 0.5 mM IPTG, and the CYP3A4 construct was expressed for 48 72 h before cells were lysed by sonication; expression levels were quantified by reduced CO binding.
That identity was expressed, for a time, in Trump's biography on his corporate website.
Citizen support was expressed for agricultural heritage (P < 0.001) and biodiversity-rich dry grasslands (P < 0.001).
The highest preference was expressed for wildland scenes containing water, followed by cultural landscapes and traditional farm environments.
When concern was expressed for slaves, it was for their good care, not for their unfree status.
In a booklet handed out at the commemoration, Hollande wrote: "The recognition of this fault was expressed for the first time, with lucidity and courage, by Jacques Chirac on July 16 , 1995
"We are angered by this letter of 'support', because no support was expressed for the female complainant or for the other female students who felt it was safe to make complaints after Steven Galloway was suspended".
The discussion was described as cordial in a statement by the Holy See, which said "satisfaction was expressed" for the good US relationship with the Vatican and their "joint commitment in favour of life, and freedom of worship and conscience".
In the conventions of some of the states which ratified the constitution a desire was expressed for amendments declaratory of the rights of the people and restrictive of the powers of the new government, in order, as stated at the time, to prevent misconception or abuse of its powers.
Gratitude was expressed for the humanitarian work done in Albania by the Red Cross and the Rockefeller Foundation in the fields of public health and in eradication of malaria.
One of the main findings of the project was a 'preference hierarchy' in respect of policy methods, according to which the strongest support was expressed for voluntary and education-based tools, followed by market-based instruments, with command-and-control regulation identified as a measure of 'last resort'.
