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Mitochondrial membrane potential was evaluated by staining with rhodamine 123.
Cell viability was evaluated by staining with propidium iodide for dead cells and fluorescein diacetate for live cells.
The growth of cells was evaluated by staining with α-Sarcomeric actin (α-SCA) and Troponin T type 2 (TNNT2), and the viability of cardiomyocytes was studied in vitro by assessing the expression levels of several cardiac ion channels, including CACNL1A1, Connexin 43 and SCN5A.
The structure of the cornea prior to culture (0 h) and at 7, 14, and 18 days in culture was evaluated by staining with hematoxylin/eosin, and by ultrastructural analysis that included a morphometric study of the type and number of adhesion complexes.
Acrosomal status was evaluated by staining with FITC-PSA according to Mendoza C et al., [9].
Viral replication was evaluated by staining with 600 µl of staining solution (6.6 mM potassium ferrocyanide, 3.3 mM MgCl2, 0.7 mg/ml X-Gal in PBS).
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Additionally, adhesion and attachment of human fibroblasts to the scaffold were evaluated by staining with Phalloidin-Atto 565 (Sigma Aldrich and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) according to manufacturer's specifications.
The phenotype of the DC were evaluated by staining with phycoerythrin (PE -conjugated mouse anti-human antibodies: CD80 (L307.4), CD83 (HB15e), and CD86 (FUN-1).
Mitochondrial membrane potential can be evaluated by staining with rhodamine 123.
Reaction products were separated on a 2% agarose gel and were evaluated by staining with GelRed.
The PCR products were electrophoresed on 1.5% agarose gels and their amounts were evaluated by staining with ethidium bromide.
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